Accuracy Analysis of a Next-Generation Tissue Microarray on Various Soft Tissue Samples of Wistar Rats

This study aimed to investigate accuracy in different sectional planes of the TMA Grand Master (3DHISTECH) Workstation various soft tissue samples collected from Wistar rats. A total 108 animals were sacrificed and 963 specimens 12 soft-tissue types. 3307 cores punched transferred into 40 recipient blocks. Digital image analysis was performed. Core loss showed a significant correlation with type highest skin (p < 0.001), renal medulla femoral artery, nerve, vein bundle 0.01). Overall, 231 (7.0%) lost. Hit rate performed 1852 punches. The target hit completely, partially missed totally by 89.4%, 7.2% 2.2%. 54.5% punches had good less than 200 µm deviation centre targeted region 92.6% 500 µm. Accuracy decreases greater depth. In deepest plane roughly 0.5 mm median depth, almost 90% below Recommendations for automated creation are given this article. ngTMA®-method has proven accurate reliable tissues, even deeper layers.